RAPID COMMUNICATION Species-Diagnostic Single-Nucleotide Polymorphism and Sequence-Tagged Site Markers for the Parasitic Wasp Genus Nasonia (Hymenoptera: Pteromalidae)

نویسندگان

  • O. NIEHUIS
  • A. K. JUDSON
  • J. H. WERREN
  • W. B. HUNTER
  • P. M. DANG
  • S. E. DOWD
  • B. GRILLENBERGER
  • L. W. BEUKEBOOM
  • J. GADAU
چکیده

Wasps of the genus Nasonia are important biological control agents of house ßies and related Þlth ßies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identiÞcation have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and wemapped them in theNasonia genome. The markers are species-diagnostic and evenly spread over all Þve chromosomes. They are ideal for rapid species identiÞcation and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.

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تاریخ انتشار 2007